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Publikationen

Hochholdinger F, Guo L, Rosignoli S, Rasmussen MW, Suresh K, Sangiorgi G, Camerlengo F, ; Zeisler-Diehl VV, Schreiber L, Dockter C, Pauly M, Tuberosa R, Salvi S, 2025, HvGLU3 encodes a putative endo-1,4-β-D-glucanase required for cellulose biosynthesis in barley root, Plant Physiology, doi: https://doi.org/10.1093/plphys/kiaf311

Beyer HM, Ramirez V, 2024, Integrating bioprinting and optogenetic technologies for presision plant tissue engineering, Current opinion in biotechnology, doi: 10.1016/j.copbio.2024.103193

Robertz S, Philipp M, Schipper K, Richter P, Miebach K, Magnus J, Pauly M, Ramirez V, 2024, Monitoring corn stover processing by the fungus Ustilago maydis, Bioresources and Bioprocessing, 11 (87), doi: 10.1186/s40643-024-00802-3

Gutschker S, Ruescher D, Rabbi IY, Rosado-Souza L, Pommerrenig B, Pauly M, Robertz S, von Doorn AM, Schlereth A, Neuhaus HE, Fernie AR, Reinert S, Sonnewald U, Zierer W, 2024, Carbon usage in yellow-fleshed Manihot esculenta storage roots shifts from starch biosynthesis to cell wall and raffinose biosynthesis via the myo-inositol pathway, Plant Journal, 119, 2045-2062, doi: 10.1111/tpj.16909

Huebbers JW, Caldarescu GA, Kubatova Z, Sabol P, Levecque SCJ, Kuhn H, Kulich I, Reinstädtler A, Büttgen K, Manga-Robles A, Melida H, Pauly M, Panstruga R, Zarsky V, 2023, Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew, Plant Cell, doi.org/10.1093/plcell/koad319

Wanke A, van Boerdonk S, Mahdi LK, Wawra S, Neidert M, Chandrasekar B, Saake P, Saur IML, Derbyshire P, Holton N, Menke FLH, Brands M, Pauly M, Acosta IF, Zipfel C, Zuccaro A, 2023, A GH81-type b-glucan binding protein enhances colonization by mutualistic fungi in barley, Current Biology 33,1-14, doi: 10.1016/j.cub.2023.10.048

Immelmann R, Gawenda N, Ramirez V, Pauly M, 2023, Identification of a xyloglucan beta-xylopyranosyltransferase from Vaccinium corymbosum. Plant Direct 7e514, doi: 10.1002/pld3.514

Wang S, Robertz S, Seven M, Kraemer F, Kuhn BM, Liu Lifeng, Lunde C, Pauly M, Ramirez V, 2023, A large-scale forward genetic screen for maize mutants with altered lignocellulosic properties. Frontiers in Plant Science, 14:1099009 doi: 10.3389/fpls.2023.1099009

Behar H, Mottiar Y, Chandrasekhar R, Grappadelli AC, Pauly M, Samuels AL, Mansfield SD, Brumer H, 2023, Populus endoglucanase 16 localizes to the cell walls of developing tissues. Plant Direct, e00482. doi: 10.1002/pld3.482   

Ökmen B, Jaeger E, Schilling L, Finke N, Klemd A, Lee YO, Wemhörner R, Pauly M, Neumann U, Doehlemann G, 2022, A conserved enzyme of smut fungi facilitates cell-to-cell extension in the plant bundle sheath, Nature communications, 13 (1), 1-13, doi: https://doi.org/10.1038/s41467-022-33815-7

Perez-Anton M, Schneider I, Kroll P, Hofhuis H, Metzger S, Pauly M, Hay A, 2022, Explosive seed dispersal depends on SPL7 to ensure sufficient copper for localized lignin deposition via laccases, PNAS, 119 (24), e2202287119, doi: https://doi.org/10.1073/pnas.2202287119

Perrot T, Pauly M, Ramirez V, 2022, Emerging roles of b-glucanases in plant development and adaptive responses, Plants, 11 (9), 1119; doi: 10.3390/plants11091119 

Chandrasekar B, Wanke A, Wawra S, Saake P, Mahdi L, Charura N, Neidert M, Poschmann G, Malisic M, Thiele M, Stühler K, Dama M, Pauly M, Zuccaro A, 2022, Fungi hijack a ubiquitous plant apoplastic endoglucanase to release a ROS scavenging b-glucan decasaccharide to subvert immune responses, Plant Cell, 34 (7), 2765-2784; doi: 10.1093/plcell/koac114 

*O’Neill MA, Darvill AG, Etzler ME, Mohnen D, Perez S, Mortimer JC, Pauly M, 2022, Chapter 24 Viridiplantae and Algae, Essentials of Glycobiology. 4th edition , Varki A, Cummings RD, Esko JD, et al., editors. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press

Hübbers JW, Büttgen K, Leissing F, Mantz M, Pauly M, Hüsgen PF, Panstruga R, 2022, An advanced method for the release, enrichment, and purification of high-quality Arabidopsis thaliana rosette leaf trichome enables profound insights into the trichome proteome, Plant Methods, 18 (1), 1-23, doi: https://doi.org/10.1186/s13007-021-00836-0

Jablonowski ND, Pauly M, Dama M, 2022, Microwave assisted pretreatment of Szarvasi (Agropyron elongatum) biomass to enhance enzymatic saccharification and direct glucose production, Frontiers in Plant Science, Vol 12, Article 767254, doi:10.3389/fpls.2021.767254

Krämer FJ, Koch M, Kuhn BM, Rühl C, Brown PJ, Hoffmann P, Göhre V, Hake S, Pauly M, Ramirez V, 2021, A mixed-linkage (1,3;1,4)-b-D-glucan specific hydrolase mediates dark-triggered degradation of this plant polysaccharide, Plant Physiology, 185 (4), 1559-1573; doi:10.1093/plphys/kiab009

Robert M, Waldhauer J, Stritt F, Pauly M, Voiniciuc C, 2021, Modular biosynthesis of plant hemicellulose and its impact on yeast cells, Biotechnology for Biofuels, 14:140, doi: 10.1186/s13068-021-01985-z

Verhertbruggen Y, Bouder A, Vigouroux J, Alvarado C, Geairon A, Guillon F, Wilkinson MD, Stritt F, Pauly M, Lee MY, Mortimer JC, Scheller HV, Mitchell RAC, Voiniciuc C, Saulnier L, Chateigner-Boutin AL, 2021, The TaCslA12 gene expressed in the wheat grain endosperm synthesizes wheat-like mannan when expressed in yeast and Arabidopsis, Plant Science, 10.1016/j.plantsci.2020.110693

Weidener D, Dama M, Dietrich SK, Ohrem B, Pauly M, Leitner W, de Maria PD, Grande P, Klose H, 2020, Multiscale analysis of lignocellulose recalcitrance towards OrganoCat pretreatment and fractionation, Biotechnology for Biofuels, 13:155, doi:10.1186/s13068-020-01796-8

Kim SJ, Chandrasekar B, Rea A, Danhof L, Zemelis-Durfee S, Thrower N, Shepard Z, Pauly M, Brandizzi F, Keegstra K, 2020, The synthesis of xyloglucan, an abundant plant cell wall polysaccharide, requires CSLC function, PNAS, 117 (33), 20316-20324, doi:10.1073/pnas.2007245117

Sinclair SA, Gille S, Pauly M, Krämer U, 2020, Regulation of acetylation of plant cell wall components is complex and responds to external stimuli, Plant Signaling and Behavior, 15 (1), 1678185, doi:10.1080/15592324.2019.1687185

Pauly M, Gawenda N, Wagner C, Fischbach P, Ramirez V, Axmann IM, Voiniciuc C, 2019, The suitability of orthogonal hosts to study plant cell wall biosynthesis, Plants, 8, 516, doi:10.3390/plants8110516

Grande PM, Weidener D, Dietrich S, Dama M, Bellof M, Maas R, Pauly M, Leitner W, Klose H, de Maria PD, 2019, Organocat fractionation of empty fruit bunches from palm trees into lignin, sugars, and cellulose-enriched pulp, ACS Omega, in press, doi: 10.1021/acsomega.9b01371

Ramirez V, Pauly M, 2019, Genetic dissection of cell wall defects and the strigolactone pathway in Arabidopsis, Plant Direct 3 (6), e00149, doi: 10.1002/pld3.149

Voiniciuc C, Dama M, Gawenda N, Stritt F, Pauly M, 2019, Mechanistic insights from plant heteromannan synthesis in yeast, Proceedings of the National Academy of the USA, 116 (2), 522-527, https://doi.org/10.1073/pnas.1814003116

Llerena JPP, Figueiredo R, Brito MdS, Kiyota E, Mayer JLS, Araujo P, Schimpl FC, Dama M, Pauly M, Mazzafera P, 2019, Deposition of lignin in four species of Saccharum, doi: 10.1038/s41598-019-42350-3, Scientific Reports, 9:5877, doi: 10.1038/s41598-019-42350-3

Polko JK, Barnes WJ, Voiniciuc C, Doctor S, Steinwand B, Hill Jr JL, Tien M, Pauly M, Anderson CT, Kieber JJ, 2018, SHOU4 proteins regulated trafficking of cellulose synthase complexes to the plasma membrane, Current Biology, 28:19, 3174-3182, doi.org/10.1016/j.cub.2018.07.076

Pauly M and Ramirez V, 2018, New insights in wall polysaccharide O-acetylation, Frontiers in Plant Sciences 9: 1210, doi.org/10.3389/fpls.2018.01210

van der Schuren A, Voiniciuc C, Bragg J, Ljung K, Vogel J, Pauly M, Hardtke C, 2018 Broad spectrum developmental role of Brachypodium AUX1, New Phytologist, 219 (4), 1216-1223, doi.org/10.1111/nph.15332

Xia J, Zhao Y, Burks P, Pauly M, Brown PJ, 2018, A Sorghum NAC gene is associated with variation in vascular development, biomass properties, and yield potential, Plant Direct, 2 (7) e00070, doi.org/10.1002/pld3.70

Ramirez V, Guangyan X, Mashigushi K, Yamaguchi S, Pauly M, 2018, Growth- and stress related defects associated to wall hypoacetylation are strigolactone dependent, Plant Direct, 2 (6), e00062, doi.org/10.1002/pld3.62

Persson S, Sørensen I, Moller I, Willats W, Pauly M, 2018, Dissection of plant cell walls by high-throughput methods, Annual Plant Reviews online, doi.org/10.1002/9781119312994.apr0431

Voiniciuc C, Pauly M, Usadel B, 2018, Monitoring polysaccharide dynamics in the plant cell wall, Plant Physiology, 176: 2590-2600, doi.org/10.1104/pp.17.01776

Zhu L, Dama M, Pauly M, 2018, Identification of an arabinopyranosyltransferase from Physcomitrella patens involved in the synthesis of the hemicellulose xyloglucan, Plant Direct, 2 (3) e00046, doi.org/10.1002/pld3.46

Hsia M, O'Malley R, Cartwright A, Nieu R, Gordon S, Kelly S, Williams T, Woods D, Zhao Y, Bragg J, Jordan M, Pauly M, Ecker J, Gu Y, Vogel J, 2017, Sequencing and functional validation of the JGI Brachypodium distachyon T-DNA collection, Plant Journal, 91:3 361-370, doi.org/10.1111/tpj.13582

Møller S, Yi X, Velásquez SM, Gille S, Malik PL, Poulsen C, Olsen C, Rejzek M, Parsons H, Zhang Y, Wandall H, Clausen H, Field R, Pauly M, Estevez J, Harholt J, Ulvskov P, Petersen B, 2017, Identification and evolution of a plant cell wall specific glycoprotein glycosyltransferase, ExAD, Scientific Reports 7:45341, doi: 10.1038/srep45341 

Rosa M, Abraham J, Lewis MW, Fonseca JP, Tian W, Ramirez V, Luan S, Pauly M, Hake S, 2017 Narrow odd dwarf, the maize MID-COMPLEMENTING ACTIVITY ortholog, coordinates organ growth and tissue patterning, Plant Cell, 29:474-490, DOI: 10.1105/tpc.16.00878 

Gao Y, He C, Zhang D, Liu X, Xu Z, Yanbao T, Liu X-H, Zhang S, Pauly M, Zhou Y, Zhang B, 2017, Two trichome birefringence-like proteins mediate xylan acetylation, which is essential for leaf blight resistance in rice, Plant Physiology, 173(1): 470-481, doi: 10.1104/pp.16.01618 

Salinas C, Handford M, Pauly M, Cardemil L, 2016, Structural modifications of fructans in Aloe barbadensis, PloS One, 11(7):e0159819 DOI 10.1371/journal.pone.0159819

Rautengarten C, Ebert B, Liu L, Smith-Moritz AM, Pauly M, Orellana A, Scheller HV, Heazlewood JL, 2016, The Arabidopsis Gogli-localized GDP-fucose transporter is required for normal plant development, Nature Communications, 7:12119 DOI 10.1038/ncomms12119

Stonebloom S, Ebert B, Xiong G, Lao J,  Pauly M, Heazlewood JL, Scheller HV, 2016, A DUF-246 family glycosyltransferase-like protein affects the biosynthesis of rhamnogalacturonan-I arabinogalactans, BMC Plant Biology, 16:90 DOI 10.1186/s12870-016-0780-x

Pauly M, Keegstra K, 2016, Biosynthesis of the plant cell wall matrix polysaccharide xyloglucan, Annual Review of Plant Biology, 67(1): 235-259, doi: 10.1146/annurev-arplant-043015-112222 

Bethke G, Thao A, Xiong G, Li B, Soltis NE, Hatsugai N, Hillmer RA, Katagiri F, Kliebenstein DJ, Pauly M, Glazebrook J, 2016, Pectin biosynthesis is critical for cell wall integrity and immunity in Arabidoposis, Plant Cell, 28: 537-556, doi: 10.1105/tpc.15.00404 

Liu L, Hsia MM, Dama M, Vogel J, Pauly M, 2016, A xyloglucan backbone 6-O-acetyltransferase from Brachypodium distachyon modulates xyloglucan xylosylation, Molecular Plant, 9: 615-617, doi:10.1016/j.molp.2015.11.004 

Liberatore KL, Xu C, MacAlister CA, Jiang K, Brooks C, Ogawa-Ohnishi M, Xiong G, Pauly M, Matsubayashi Y, Lippman ZB, 2015, A cascade of arabinosyltransferases controls stem cell homeostasis in tomato, Nature Genetics, 47: 784-792, doi: 10.1038/ng.3309 

Loque D, Scheller HV, Pauly M, 2015, Engineering of plant cell walls for enhanced biofuel production, Current opinion in Plant Biology, 25: 151-161, doi:https://doi.org/10.1016/j.pbi.2015.05.018 

De Souza A, Pauly M, 2015, Comparative genomics of pectinacetylesterases: insights on function and biology, Plant Signal and Behavior, 10:9 e1055434, DOI: 10.1080/15592324.2015.1055434 

Yang F, Afzal W, Cheng K, Liu N, Pauly M, Bell AT, Liu Z, Prausnitz JM, 2015, Nitric-acid hydrolysis of miscanthus giganteus to fermentable sugars, Biotechnology and Bioprocess Engineering, 20 (2): 304-314, doi: https://doi.org/10.1007/s12257-014-0658-4

Liu L, Paulitz J, Pauly M, 2015, The presence of fucogalactoxyloglucan and its synthesis in rice indicates conserved functional importance in plants, Plant Physiology 168: 549-560, doi: https://doi.org/10.1104/pp.15.00441

Draeger C, Fabrice TN, Mouille G, Kuhn B, Moller I, Abdou MT, Frey B, Pauly M, Bacic T, Ringli C, 2015, Arabidopsis LEUCINE-RICH REPEAT EXTENSIN (LRX) proteins modify cell wall composition and influence plant growth, BMC Plant Biology 15: 155-166, doi: https://doi.org/10.1186/s12870-015-0548-8

Xiong G, Dama M, Pauly M, 2015, Glucuronic acid moieties on xylan are functionally equivalent to O-acetyl-substituents, Molecular Plant 8: 1119-1121, doi:10.1016/j.molp.2015.02.013

Schultink A, Naylor D, Dama M, Pauly M, 2015, The role of the plant specific AXY9 gene in plant cell wall polysaccharide O-acetylation, Plant Physiology, 167 (4):1271-1283, doi: 10.1104/pp.114.256479

Mansoori N, Schultink A, Schubert J, Pauly M, 2015, Expression of heterologous xyloglucan xylosyltransferases in Arabidopsis to investigate their role in determining xyloglucan xylosylation substitution patterns, Planta, 241:1145-1158, doi: 10.1007/s00425-015-2243-2 

Ben-Toy D, Abraham Y, Stav S, Thompson K, Loraine A, Elbaum R, Souza A, Pauly M, Kieber JJ, Harpaz-Saad S, 2015, COBRA-LIKE 2, a member of the GPI-anchored COBRA-LIKE family, plays a role in cellulose deposition in Arabidopsis seed coat mucilage, Plant Physiology, 167 (3), 711-724, doi: https://doi.org/10.1104/pp.114.240671

Taylor-Teeples M, Lin L, Trabucco GM, de Lucas M, Turco G, Doherty C, Toal TW, Gaudinier A, Young NF, Xiong G, Corwin J, Tsoukalas N, Pauly M, Kliebenstein DJ, Tagkopoulos I, Breton G, Ahnert S, Kay SA, Brady SM, Hazen SP, 2015, Environmental, developmental and genotype-dependent regulation of xylem cell specification and secondary cell wall biosynthesis in Arabidopsis thaliana, Nature, 517, 571-575, doi: https://doi.org/10.1038/nature14099

Ebert B, Rautengarten C, Guo X, Xiong G, Herter T, Chan LJG, Adams PD, Petzold CJ, Pauly M, Willats WGT, Heazlewood JL, Scheller HV, 2014, Identification and characterization of a Golgi localized UDP-xylose transporter family from Arabidopsis, Plant Cell, 27 (4):1218-1227, doi: 10.1105/tpc.114.133827  

Schultink A, Liu L, Zhu L, Pauly M, 2014, Structural diversity and function of xyloglucan sidechain substituents, Plants, 3, 526-542, doi: 10.3390/plants3040526 

de Souza A, Hull PA, Gille S, Pauly M, 2014, Identification and characterization of the distinct plant pectin esterases PAE8 and PAE9 and its mutants, Planta, 240 (5) 1123-1138, doi: 10.1007/s00425-014-2139-6 

Rautengarten C, Ebert B, Moreno I, Temple H, Herter T, Link B, Doñas D, Moreno A, Saéz-Aguayo S, Blanco MF, Mortimer J, Schultink A, Reiter WD, Dupree P, Pauly M, Heazlewood JL, Scheller HV, Orellana A, 2014, Identification of six Golgi localized bi-functional UDP-Rhamnose / UDP-Galactose transporters in Arabidopsis, PNAS, 111 (31) 11563-11568, doi: https://doi.org/10.1073/pnas.1406073111

Liu Z, Padmanabhan S, Cheng K, Xie H, Gokhale A, Na H, Pauly M, Bell AT, Prausnitz JM, 2014, Two-step delignification of Miscanthus to enhance enzymatic hydrolysis: Aqueous ammonia followed by sodium hydroxide and oxidants, Energy and Fuels, 28, 542-548, doi: https://doi.org/10.1021/ef401317z

Knoch E, Dilokpimol A, Tryfona T, Poulsen CP, Xiong G, Harholt J, Peterson BL, Ulvskov P, Hadi MZ, Kotake T, Tsumuraya Y, Pauly M, Dupree P, Geshi N, 2013, A b-glucuronosyl-transferase from Arabidopsis thaliana involved in the biosynthesis of type II arabinogalactan has a role in cell elongation during seedling growth, Plant Journal, 76 (6), 1016-1029, doi: 10.1111/tpj.12353 

Manabe Y, Verhertbruggen Y, Gille S, Harbolt J, Chong SL, Pawar P, Mellerowicz E, Tenkanen M, Cheng K, Pauly M, Scheller HV, 2013, RWA proteins play vital and distinct roles in cell wall O-acetylation in Arabidopsis thaliana, Plant Physiology, 163, 1107-1117, doi: 10.1104/pp.113.225193

Schultink A, Cheng K, Yung-Bom K, Cosgrove D, Pauly M, 2013, The identification of two arabinosyltransferases from tomato reveals functional redundancy of xyloglucan side-chain substituents, Plant Physiology, 163, 86-94, doi: 10.1104/pp.113.221788 

Pauly M, Gille S, Liu L, Mansoori N, de Souza A, Schultink A, Xiong G, 2013, Hemicellulose biosynthesis, Planta, 238 (4), 627-642, doi: 10.1007/s00425-013-1921-1 

Koenig D, Jimenez-Gomez JM, Kimura S, Fulop D, Chitwood DH, Headland LR, Kumar R, Covington MF, Devisetty UK, Tat AV, Tohge T, Bolger A, Schneeberger K, Ossowski S, Lanz C, Xiong G, Taylor-Teeples M, Brady SM, Pauly M, Weigel D, Usadel B, Fernie AR, Peng J, Sinha NR, Maloof JN, 2013,Comparative transcriptomics in wild and domesticated tomato, PNAS, 110 (28) E2655-E2662, doi: https://doi.org/10.1073/pnas.130960611

Xiong G, Cheng K, Pauly M, 2013, Reduction in xylan O-acetylation results in increased recalcitrance to saccharification as indicated by the Arabidopsis mutant tbl29, Molecular Plant, 6 (4) 1373-1375, doi:10.1093/mp/sst014

Gille S, Sharma V, Baidoo EEK, Keasling JD, Scheller HV, Pauly M, 2013, Arabinosylation of an AGP-like polymer impacts root growth as exemplified by the Arabidopsis glycosyltransferase mutant ray1, Molecular Plant, 6 (4) 1369-1372, doi: 10.1093/mp/sst029 

Cheng K, Sorek H, Zimmermann H, Wemmer DE, Pauly M, 2013, Solution-state 2D NMR spectroscopy of plant cell walls enabled by a DMSO-d6[Emim]OAc solvent, Analytical Chemistry, 85 (6) 3213-3221, doi: 10.1021/ac303529v 

Liu Z, Padmanabhan S, Cheng K, Xie H, Schwyter P, Pauly M, Bell AT, Prausnitz JM, 2012, Aqueous-ammonia delignification of miscanthus and enzymatic hydrolysis to sugars, Bioresource Technology, 135 23-29, doi: https://doi.org/10.1016/j.biortech.2012.10.133

Handford M, Furlán CR, Marchant L, Segura M, Gómez D, Alvarez-Buylla E, Xiong G, Pauly M, Orellana A, 2012, Arabidopsis plants lacking AtUTr7, a Golgi-localized UDP-glucose/UDP-galactose transporter, exhibit alterations in lateral roots, Molecular Plant, 5 (6) 1263-1280, doi: 10.1093/mp/sss074 

Park SH, Mei C, Pauly M, Ong RG, Dale BE, Sabzikar R, Fotoh H, Nguyen T, Sticklen M, 2012, Down regulation of maize cinnamoyl-CoA reductase via RNAi technology creates brown midrib and improves AFEX-pretreated conversion into fermentable sugars for biofuels, Crop Science 52 (6) 2687-2701, doi: https://doi.org/10.2135/cropsci2012.04.0253

Chiniquy D, Sharma V, Schultink A, Baidoo EE, Rautengarten C, Cheng K, Carroll A, Ulvskov P, Harholt J, Keasling JD, Pauly M, Scheller HV, Ronald PC, 2012, XAXT1, a grass specific xylan:xylosyltransferase in the glycosyltransferase family 61, Proceedings of the National Academy of the USA 109 (42) 17117-17122, doi: https://doi.org/10.1073/pnas.1202079109

Jensen JK, Schultink A, Keegstra K, Wilkerson CG, Pauly M, 2012, RNA-Seq. of developing nasturtium seeds (Tropaeolum majus): Identification and characterization of an additional glactosyltransferase involved in xyloglucan biosynthesis, Molecular Plant, 5 (5) 984-992, doi: https://doi.org/10.1093/mp/sss032

Gille S, Pauly M, 2012, Mechanism of plant cell wall O-acetylation, Frontiers in Plant Physiology 3:12, doi: 10.3389/fpls.2012.00012

Gille S, Souza A, Xiong G, Benz M, Schultink A, Ida-Reca B, Pauly M, 2011, O-acetylation ofxyloglucan requires AXY4/AXY4L, proteins with a TBL and DUF231 domain, Plant Cell 23 (11) 4041-4053, doi: 10.1105/tpc.111.091728 

Chuck G, Tobias C, Kraemer F, Sun L, Li C, Arora R, Singh S, Dibble D, Vogel J, Simmons B, Pauly M, Hake S, 2011, Overexpression of the maize conrgrass1 microRNA gene prevents flowering, improves digestability and increases starch content of biofuel crop plants, Proceedings of the National Academy of the USA 108 (42) 17550-17555, doi: https://doi.org/10.1073/pnas.1113971108

Gunl M, Neumetzler L, Kraemer F, Souza A, Schultink A, Pena M, York WS, Pauly M, 2011, AXY8 encodes an a-fucosidase, underpinning the importance of apoplastic metabolism on the fine structure of plant cell wall polysaccharides, Plant Cell 23 (11), 4025-4040, doi: 10.1105/tpc.111.089193 

Troncoso-Ponce MA, Kilaru A, Cao X, Durrett T, Fan J, Jensen J, Pauly M, Wilkerson C, Ohlrogge J, 2011, Comparative deep transcriptional profiling of four developing oilseeds, Plant Journal 68, 1014-1027, doi: 10.1111/j.1365-313X.2011.04751.x 

Gille S, Kun C, Skinner ME, Liepman AH, Wilkerson C, Pauly M, 2011, Deep Sequencing of Voodoo Lily (Amorphophallus konjac): An approach to identify relevant genes involved in the synthesis of the hemicellulose glucomannan, Planta, 234, 515-526, doi: doi.org/10.1007/s00425-011-1422-z

Velasquez SM, Ricardi MM, Dorosz JG, Fernandez PV, Nadra AD, Pol-Fachin L, Egelund J, Gille S, Ciancia M, Verli H, Pauly M, Bacic A, Olsen CE, Ulvskov P, Peterson BL, Somerville C, Iusem ND, Estevez JM, 2011, O-glycosylated cell wall proteins are essential in polarized root hair growth, Science, 332, 1401-1403, 10.1126/science.1206657 

Manabe Y, Nafisi M, Verhertbruggen Y, Orfila C, Gille S, Rautengarten C, Cherk C, Marcus SE, Somerville S, Pauly M, Knox JP, Sakuragi Y, Scheller HVS, 2011, Loss-of-function mutation of reduced wall acetylation 2 in Arabidopsis leads to reduced cell wall acetylation and increased resistance to Botrytis cinerea, Plant Physiology, 155 (3), 1068-1078, doi: 10.1104/pp.110.168989 

Gunl M, Pauly M, 2011, AXY3 encodes a a-xylosidase that impacts the abundance and accessibility of the hemicellulose xyloglucan in Arabidopsis plant cell walls, Planta, 233 (4), 707-719, doi: 10.1007/s00425-010-1330-7

Gunl M, Kraemer FJ, Pauly M, 2011, Oligosaccharide mass profiling (OLIMP) of cell wall polysaccharides by MALDI-TOF/MS, Methods in Molecular Biology Vol 715: The Plant Cell Wall, 43-54, Editor Popper Z, Humana Press, doi: 10.1007/978-1-61779-008-9_3 

Perrson S, Sorensen I, Moller I, Willats W, Pauly M, 2011, Dissection of the Plant Cell Wall by High Throughput Methods, Annual Plant Reviews Vol 41: Plant Polysaccharides: Biosynthesis and Bioengineering, 43-64, Editor Ulvskov P, Wiley-Blackwell, doi: https://doi.org/10.1002/9781119312994.apr0431

Suen G, Scott JJ, Aylward FO, Adams SM, Tringe SG, Pinto-Tomas AA, Foster CE, Pauly M, Weimer PJ, Barry KW, Goodwin LA, Bouffard P, Li L, Osterberger J, Harkins TT, Slater SC, Donohue TJ, Currie CR, 2010, An insect herbivore microbiome with high plant biomass degrading capacity, PLoS Genetics, 6 (9), 433-443, doi: https://doi.org/10.1371/journal.pgen.1001129

Pauly M, 2010, A blue-print of the protoplast’s dwelling, Plant Physiology, Classic collection, 154, 1

Kaida R, Serada S, Norioko N, Norioka S, Neumetzler L, Pauly M, Sampedro J, Zarra I, Hayashi T, Kaneko TS, 2010, Potential role for purple acid phosphatase in the dephosphorylation of wall proteins in tobacco cells, Plant Physiology, 153 (2), 603-610, doi: 10.1104/pp.110.154138

Lopes FJF, Pauly M, Lau EY, Diola V, Passos JL, Loureiro ME, Brommonshenkel SH, 2010, The EgMUR3 xyloglucan galactosyltransferase from Eucalyptus grandis complements the mur3 cell wall phenotype in Arabidopsis thaliana, Tree Genetics and Genomes, 6, 745-756, doi: https://doi.org/10.1007/s11295-010-0288-8

Gunl M, Gille S, Pauly M, 2010, OLIigo Mass Profiling (OLIMP) of extracellular polysaccharides, in production in Journal of visualized Experiments Journal of visualized Experimentshttp://www.jove.com/index/details.stp?id=2046, doi: 10.3791/2046

Pauly M, Keegstra K, 2010, Plant cell walls as precursors for biofuels, Current Opinion in Plant Biology, 13 (3), 305-312, doi: https://doi.org/10.1016/j.pbi.2009.12.009

Santoro N, Cantu SL, Tornqvist CEI, Falbel TG, Bolivar JL, Patterson SE, Pauly M, Walton JD, 2010, A high throughput platform for screening milligram quantities of plant biomass for lignocellulosic digestability, BioEnergy Research, 3 (1), 93-102, doi: https://doi.org/10.1007/s12155-009-9074-6

Foster CE, Martin T, Pauly M, 2010, Comprehensive compositional analysis of Plant Cell Walls (lignocellulosic biomass); Part II: carbohydrates, Journal of visualized Experiments, 37, http://www.jove.com/index/details.stp?id=1837, doi: 10.3791/1837

Foster CE, Martin T, Pauly M, 2010, Comprehensive compositional analysis of Plant Cell Walls (lignocellulosic biomass); Part I: lignin, Journal of visualized Experiments, 37; http://www.jove.com/index/details.stp?id=1745, doi: 10.3791/1745

Gille S, Haensel U, Ziemann M, Pauly M, 2009, Identification of plant cell wall mutants by means of a forward chemical genetic approach using hydrolases, Proc. Nat. Academy Sciences U.S.A. 106 (34), 14699-14704, doi: https://doi.org/10.1073/pnas.0905434106

Li M, Xiong G, Li R, Tan D, Zhang B, Cui J, Pauly M, Cheng Z, Zhou Y, 2009, Rice Cellulose Synthase-Like D4 Is Essential for Normal Cell Wall Biosynthesis and Plant Growth, Plant Journal 60, 1055-1069, doi: 10.1111/j.1365-313X.2009.04022.x 

Obel N, Erben V, Schwarz T, Kuehnel S, Fodor A, Pauly M, 2009, Microanalysis of plant cell wall polysaccharides, Molecular Plant 2 (5), 922-932, doi: 10.1093/mp/ssp046 

Abasolo W, Eder M, Yamauchi K, Obel N, Reinecke A, Neumetzler L, Dunlop JWC, Mouille G, Pauly M, Hoefte H, Burgert I, 2009, Pectins may hinder the unfolding of xyloglucan chains during cell elongation – implications of the mechanical performance of Arabidopsis hypocotyls with pectin alterations, Molecular Plant 2 (5), 990-999, doi: 10.1093/mp/ssp065 

Pauly M, Keegstra K, 2008, Tear down this wall, Current Opinion in Plant Biology 11 (3), 233-235, doi: 10.1016/j.pbi.2008.04.002 

Cavalier DM, Lerouxel O, Neumetzler L, Yamauchi K, Reinecke A, Freshour G, Zabotina O, Hahn MG, Burgert I, Pauly M, Raikhel N, Keegstra K, 2008, Disruption of two Arabidopsis thaliana xylosyltransferase genes results in plants deficient in xyloglucan, a major primary cell wall component, Plant Cell 20:1519-1537, doi: 10.1105/tpc.108.059873 

Jensen J, Sorensen S, Harholt J, Geshi N, Sakuragi Y, Moller I, Zandleven J, Bernal AJ, Jensen NB, Sorensen C, Pauly M, Beldman G, Willats WGT, Scheller HV, 2008, Identification of a xylogalacturonan xylosyltransferase involved in pectin biosynthesis in Arabidopsis, Plant Cell 20: 1289-1302, doi: 10.1105/tpc.107.050906 

Wen F, Rhodesia MC, Nguyen T, Zeng, W, Keegstra K, Immerzeel P, Pauly M, Hawes MC, 2008, Inducible expression of Pisum sativum xyloglucan fucosyltransferase in the pea root cap meristem, and effects of antisense mRNA expression on root cap cell wall structural integrity, Plant Cell Reports 27:1125-1135, doi: 10.1007/s00299-008-0530-0 

Pauly M, Keegstra K, 2008, Cell wall carbohydrates and their modification as raw materials for biofuels, Plant Journal 54, 559-568, doi: 10.1111/j.1365-313X.2008.03463.x 

Leboeuf E, Immerzeel P, Gibon, Y, Steup M, Pauly M, 2008, High throughput functional assessment of polysaccharide-active enzymes using MALDI-TOF mass spectrometry as exemplified on plant cell wall polysaccharides, Analytical biochemistry, 373 (1):9-17, doi: https://doi.org/10.1016/j.ab.2007.10.007

Bernal AJ, Jensen JK, Harholt J, Sorensen S, Moller I, Blaukopf C, Johansen B, de Lotto R, Pauly M, Scheller HV, Willats WGT, 2007, Disruption of AtCSLD5 results in reduced growth, reduced xylan and homogalacturonan synthase activity and altered xylan occurrence in Arabidopsis, Plant Journal, 52, 791-802, doi: 10.1111/j.1365-313X.2007.03281.x 

Rösti J, Barton CJ, Albrecht S, Dupree P, Pauly M, Findlay K, Roberts K, Seifert GJ, 2007, UDP-glucose 4 epimerase isoforms UGE2 and UGE4 cooperate in providing UDP-galactose for cell wall biosynthesis and growth of Arabidopsis thaliana, Plant Cell 19:1565-1579, doi: 10.1105/tpc.106.049619 

Egelund J, Obel N, Ulvskov P, Geshi N, Pauly M, Bacic A, Peterson BL, 2007, Molecular characterization of two Arabidopsis thaliana glycosyltransferase mutants, rra1 and rra2, which have a reduced residual arabinose content in a polymer tightly associated with the cellulosic wall residue, Plant Molecular Biology 64(4): 439-451, doi: https://doi.org/10.1007/s11103-007-9162-y

Krupkova E, Immerzeel P, Pauly M, Schmülling T, 2007, The tumorous shoot development2 gene of Arabidopsis encoding a putative methyltransferase is required for cell adhesion and co-ordinated plant development, Plant Journal 50: 735-750, doi: 10.1111/j.1365-313X.2007.03123.x 

Kannangara R, Branigan C, Liu Y, Penfield T, Rao V, Mouille G, Höfte H, Pauly M, Riechmann JL, Broun P, 2007, Transcription factor WIN1/SHN1 regulates cutin biosynthesis in Arabidopsis thaliana, Plant Cell 19: 1278-1294, doi: 10.1105/tpc.106.047076

Lionetti V, Railola A, Camardella L, Giovane A, Obel N, Pauly M, Favaron F, Cervone F, Bellincampi D, 2007, Overexpression of pectin methylesterase inhibitors in Arabidopsis restricts fungal infection by Botrytis cinerea, Plant Physiology 143:1871-1880, doi: 10.1104/pp.106.090803 

Obel N, Neumetzler L, Pauly M, 2007, Hemicelluloses and cell expansion, in The expanding cell, Springer publishing, Verbelen C, Vissenberg K eds. p 57-88, doi: https://doi.org/10.1007/7089_2006_071

Carrari F, Baxter C, Usadel B,  Urbanczyk-Wochniak E, Zanor MI, Nunes-Nesi A, Nikiforova V, Centero D, Ratzka A, Pauly M, Sweetlove LJ, Fernie AR, 2006, Integrated analysis of metabolite and transcript levels reveals the metabolic shifts that underlie tomato fruit development and highlight regulatory aspects of metabolic network behavior, Plant Physiology 142 (4):1380-1396, doi: 10.1104/pp.106.088534 

Bosca S, Barton CJ, Taylor NG, Ryden P, Neumetzler L, Pauly M, Roberts K, Seifert GJ, 2006, Interactions between MUR10/CesA7-dependent secondary cellulose biosynthesis and primary cell wall structure. Plant Physiology 142:1353-1363., doi: 10.1104/pp.106.087700 

Immerzeel P, Pauly M, 2006, Profiling methods for the analysis of cell wall polysaccharides, New Zealand Journal of Forestry research, 36 (1):145-156

Mouille G, Witucka-Wall H, Bryant MP, Loudet O, Rihouey C, Lerouxel O, Lerouge P, Hoefte H, Pauly M, 2006, QTL analysis of primary cell wall composition in Arabidopsis thaliana, Plant Physiology, 141 (7):1035-1044, doi: 10.1104/pp.106.079384

Diet A, Link B, Seifert GJ, Schellenberg B, Wagner U, Pauly M, Reiter WD, Ringli C, 2006, The  Arabidopsis root hair cell wall formation mutant lrx1 is suppressed by mutations in the RHM1 gene encoding a UDP-L-rhamnose synthase, Plant Cell, 18, 1630-1641, doi: 10.1105/tpc.105.038653 

Harholt J, Jensen JK, Sorensen SO, Orfila C, Pauly M, Scheller HV, 2006, Arabinan deficient 1 is a putative arabinosyltransferase involved in biosynthesis of pectin arabinan in Arabidopsis, Plant Physiology, 140 (1): 49-58, doi: 10.1104/pp.105.072744 

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renau JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D, 2006, A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of non redundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansin and plant growth, Plant Physiology, 140 (1), 30-48, doi: 10.1104/pp.105.069690 

Obel N, Erben V, Pauly M, 2006, Functional wall glycomics through oligosaccharide mass profiling, in The Science and Lore of the Plant Cell Wall: Biosynthesis, Structure and Function, Hayashi, T., ed., Brownwater press, 258-266, doi: 10.1007/978-1-61779-008-9_3 

Fettke J, Poeste S, Eckermann N, Thissen A, Pauly M, Geigenberger P, Steup M, 2005, Analysis of cytosolic heteroglycans from leaves of transgenic potato (Solanum tuberosum L) plants that under- or overexpress the pho2 phosphorylase isozyme, Plant and Cell Physiology, 46 (12), 1987-2004, doi: 10.1093/pcp/pci214 

Gibeaut DM, Pauly M, Bacic A, Fincher GB, 2005, Changes in the cell wall polysaccharides in developing barley coleoptiles, Planta, 221 (5):720-738, doi: 10.1007/s00425-005-1481-0 

Kanter U, Usadel B, Guerinaeu F, Li Y, Pauly M, Tenhaken R, 2005, The inositol oxygenase gene family of Arabidopsis is involved in the biosynthesis of nucleotide sugar precursors for cell wall matrix polysaccharides, Planta, 221 (2), 243-254, doi: https://doi.org/10.1007/s00425-004-1441-0

Usadel B, Kuschinsky AM, Steinhauser D, Pauly M, 2005, Transcriptional co-response analysis as a tool to identify new components of the wall biosynthetic machinery, Plant Biosystems, 139(1), 69-73, doi: https://doi.org/10.1080/11263500500059827

Vissenberg K, Fry SC, Pauly M, Höfte H, Verbelen H, 2005, XTH acts at the microfibril-matrix interphase during cell elongation, Journal of Experimental Botany, 56 (412): 673-683, doi: 10.1093/jxb/eri048 

Scheible WR, Pauly M, 2004, Glycosyltransferases and cell wall biosynthesis: novel players and insights, Current Opinion in Plant Biology, 7: 285-295, doi: https://doi.org/10.1016/j.pbi.2004.03.006

Fettke J, Eckermann N, Poeste S, Pauly M, Steup M, 2004, The glycan substrate of the cytosolic (pho2) phosphorylase isozyme from Pisum sativum L.: Identification, linkage analysis, and subcellular localization, Plant Journal, 39 (6), 933-946, doi: 10.1111/j.1365-313X.2004.02181.x 

Usadel B, Schlüter U, Mølhøj M, Gipmans M, Verma R, Kossmann J, Reiter WD, Pauly M, 2004, Identification and characterization of a UDP-D-glucuronate 4-epimerase in Arabidopsis, FEBS-letters, 569 (1-3), 327-331, doi: 10.1016/j.febslet.2004.06.005 

Usadel B, Kuschinsky AM, Rosso M, Eckermann N, Pauly M, 2004, RHM2 is involved in mucilage pectin synthesis and is required for the development of the seed coat in Arabidopsis thaliana, Plant Physiology, 134, 286-285, doi: 10.1104/pp.103.034314 

Eckermann N, Fettke J, Pauly M, Bazant E, Steup M, 2003, Starch-metabolism related isozymes in higher plants, in Proceedings of the 12th International Congress on Genes, Gene Families and Isozymes, Monduzzi Editore, Bologna, Italy, 241-246

Bolvig PU, Pauly M, Orfila C, Scheller HV, Schnorr K, 2003, Sequence analysis and characterization of a novel pectin acetyl esterase from Bacillus subtilis, in Advances in Pectin and Pectinase Research, Voragen, A.J.G., Schols, H., Visser, R., eds., 315-330, doi: https://doi.org/10.1007/978-94-017-0331-4_24

Pauly M, 2003, Im Dschungel der Zucker (Review), Genomxpress, 1, 3-7

Doco T, Williams P, Pauly M, O’Neill MA, Pellerin P, 2003, Polysaccharides from grape berry cell walls. Part II: Structural characterization of the xyloglucan polysaccharides, Carbohydrate Polymers, 53, 253-261, doi: https://doi.org/10.1016/S0144-8617(03)00072-9

Marry M, Cavalier DM, Schnurr JK, Netland J, Yang Z, Pezeshk V, York WS, Pauly M, White AR, 2003, Structural characterization of chemically and enzymatically derived standard oligosaccharides isolated from partially purified tamarind xyloglucan, Carbohydrate Polymers, 51, 347-356, doi: https://doi.org/10.1016/S0144-8617(02)00189-3

Choo TZ, Lerouxel O, Seveno M, Usadel B, Faye L, Lerouge P, Pauly M, 2002, Rapid structural phenotyping of plant cell wall mutants by enzymatic oligosaccharide fingerprinting, Plant Physiology, 130, 1754-1763, doi: 10.1104/pp.011965

York WS, Pauly M, Qin Q, Jia Z, Doong RL, Simon JP, Albersheim P, Darvill AG, 2002, The xyloglucan-cellulose network of plant cell walls: A prototype for the chemoenzymatic preparation of novel polysaccharide composites, Carbohydrate Bioengineering: Interdisciplinary Approaches, Teeri, T.T., Svensen, B., Gilbert, H.J., Feizi, T., eds., 143-150

Lytovchenko A, Sweetlove L, Pauly M, Fernie AR, 2002, The influence of cytosolic phosphoglucomutase on photosynthetic carbohydrate metabolism, Planta, 215, 1013-1021, doi: 10.1007/s00425-002-0826-1 

Skjøt M, Pauly M, Bush MS, Borkhardt B, McCann M, Ulvskov P, 2002, Direct interference with rhamnogalacturonan I biosynthesis in Golgi vesicles, Plant Physiology, 129 (1), 95-102, doi: 10.1104/pp.010948 

Geshi N, Pauly M, Ulvskov P, 2002, Solubilization of a galactosyltransferase that synthesizes 1,4-b-galactan sidechains in pectic rhamnogalacturonan I, Physiologia Plantarum, 114, 540-548, doi: 10.1034/j.1399-3054.2002.1140406.x 

Pauly M, Eberhard S, Albersheim P, Darvill AG, York WS, 2001, Effects of the mur1 mutation on xyloglucans produced by suspension-cultured Arabidopsis thaliana cells, Planta, 214, 67-74, doi: 10.1007/s004250100585 

Skjøt M, Kaupinnen S, Kofod LV, Fuglsang C, Pauly M, Dalbøge H, Andersen LN, 2001, Functional cloning of an endo-arabinanase from Aspergillus aculeatus and its heterologous expression in A. oryzae and tobacco, Molecular Genetics and Genomics, 265, 913-921, doi: 10.1007/s004380100489 

Pauly M, Qiang Q, Greene H, Albersheim P, Darvill AG, York WS, 2001, Changes in the structure of xyloglucan during cell elongation, Planta, 212 (5/6), 842-850, doi: 10.1007/s004250000448 

Strickland FM, Darvill A, Eberhard S, Pauly M, Albersheim P, 2001, Preservation of the delayed type hypersensitivity response to alloantigen by xyloglucans or oligogalacturonide does not correlate with the capacity to reject UV-induced skin tumors in mice, Journal of Investigative Dermatology, 116 (1), 62-68, doi: 10.1046/j.1523-1747.2001.00160.x 

Sørensen SO, Pauly M, Bush MS, Skjøt M, McCann MC, Borkhardt B, Ulvskov P, 2000, Pectin engineering: Modification of potato pectin by in vivo expression of an Endo-1,4-galactanase, Proc. Nat. Academy Sciences U.S.A., 97 (13), 7639-7644, doi: https://doi.org/10.1073/pnas.130568297

Pauly M, Porchia A, Olsen CE, Nunan KJ, Scheller HV, 2000, Enzymatic synthesis and purification of uridine diphospho-b-L-arabinopyranose, a substrate for the biosynthesis of plant polysaccharides, Analytical Biochemistry, 278, 69-73, doi: 10.1006/abio.1999.4411 

Pauly M and Scheller HV, 2000, O-acetylation of plant cell wall polysaccharides: Identification and partial characterization of a rhamnogalacturonan O-acetyl transferase from potato suspension cultured cells, Planta, 210, 659-667, doi: https://doi.org/10.1007/s004250050057

Pauly M, Albersheim P, Darvill AG, York WS, 1999, Molecular domains of the cellulose/xyloglucan network in the cell walls of higher plants, Plant Journal, 20 (6), 629-639, doi: 10.1046/j.1365-313x.1999.00630.x 

Strickland FM, Darvill AG, Albersheim P, Eberhard S, Pauly M, Pelley RP, 1999, Inhibition of UV-induced immune suppression, and interleukin–10 production by plant oligosaccharides and polysaccharides,  Photochemistry and Photobiology, 69 (2), 141-147, doi: https://doi.org/10.1111/j.1751-1097.1999.tb03266.x

Pauly M, Anderson LN, Kauppinen S, Kofod LV, York WS, Albersheim P and Darvill AG, 1999, A xyloglucan specific endo-b-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme. Glycobiology 9 (1), 93-100, doi: 10.1093/glycob/9.1.93 

Pauly M and York WS, 1998, Separation of complex carbohydrates using reversed-phase chromatography with evaporative light scattering detection (ELSD), American Biotechnology Laboratory 16 (12), 14-17

Hantus S, Pauly M, Darvill AG, Albersheim P, York WS, 1997, Structural characterization of novel L-galactose-containing oligosaccharide subunits of xyloglucans, Carbohydrate Research 304, 11-20, doi: 10.1016/s0008-6215(97)00200-0 

Zablackis E, York WS, Pauly M, Hantus S, Reiter WD, Chapple CCS, Albersheim P, Darvill A, 1996, Substitution of L-Fucose by L-Galactose in Cell Walls of Arabidopsis mur1, Science 272, 1808-1810, doi: 10.1126/science.272.5269.1808 

Pauly M, York WS, Guillen R, Albersheim P, Darvill AG, 1996, Improved protocol for the formation of N-(p-nitrobenzyloxy)aminoalditol derivatives of oligosaccharides, Carbohydrate Research 282, 1-12, doi: 10.1016/0008-6215(95)00362-2 

Guillen R, York WS, Pauly M, An J, Impallomeni G, Albersheim P, Darvill AG, 1995, Metabolism of xyloglucan generates xylose deficient oligosaccharide subunits of this polysaccharide in etiolated peas, Carbohydrate Research 277, 291-311, doi: 10.1016/0008-6215(95)00220-n 

Alpert AJ, Shukla M, Shukla AK, Zieske LR, Yuen SW, Ferguson MAJ, Mehlert A, Pauly M, Orlando R, 1994, Hydrophilic-interaction chromatography of complex carbohydrates, Journal of Chromatography A, 676, 191-202, doi: https://doi.org/10.1016/0021-9673(94)00467-6